LOCUS       pLV-[hsa-miR-548f-2]        9162 bp    DNA     circular     27-JUN-2007
SOURCE      
COMMENT     
COMMENT     This file is created by Biosettia Inc.
            http://www.biosettia.com/
FEATURES             Location/Qualifiers
     misc_feature    1807..1929
                     /label=cPPT
     CDS             7083..7943
                     /label=Amp(R)
     promoter        8144..8684
                     /label=pUC ori
     promoter        7..235
                     /label=RSV
     LTR             5688..5922
                     /label=3' LTR
     intron          526..1690
                     /label=Intron
     rep_origin      complement(6497..6952)
                     /label=f1 ori
     LTR             236..416
                     /label=5'LTR
     polyA_signal    5994..6124
                     /label=SV40 pA
     misc_feature    6125..6338
                     /label=SV40 ori
     misc_feature    5009..5605
                     /label=WPRE
     promoter        1933..2328
                     /label=human EF1a promoter
     intron          2361..3627
                     /label=intron
     CDS             3674..4999
                     /label=rPuro (RFP Puro)
     misc_RNA        3110..3417
                     /label=hsa-mir-548f-2 precursor
BASE COUNT     2209 a      2354 c      2450 g      2149 t 
ORIGIN
        1 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta 
       61 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg 
      121 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac 
      181 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc 
      241 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct 
      301 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga 
      361 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg 
      421 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact 
      481 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa 
      541 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg 
      601 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat 
      661 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc 
      721 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag 
      781 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat 
      841 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac 
      901 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat 
      961 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg 
     1021 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat 
     1081 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat 
     1141 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt 
     1201 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca 
     1261 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat 
     1321 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag 
     1381 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat 
     1441 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa 
     1501 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat 
     1561 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt 
     1621 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt 
     1681 atcgtttcag acccacctcc caaccccgag gggacccgac aggcccgaag gaatagaaga 
     1741 agaaggtgga gagagagaca gagacagatc cattcgatta gtgaacggat ctcgacggta 
     1801 tcggttaact tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt 
     1861 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaattcaa 
     1921 aattttatcg atgcctcccc gtcaccaccc cccccaaccc gccccgaccg gagctgagag 
     1981 taattcatac aaaaggactc gcccctgcct tggggaatcc cagggaccgt cgttaaactc 
     2041 ccactaacgt agaacccaga gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc 
     2101 atcactgagg tggagaagag catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca 
     2161 catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga 
     2221 gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg 
     2281 agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg 
     2341 ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta 
     2401 cgggttatgg cccttgcgtg ccttgaatta cttccacgcc cctggctgca gtacgtgatt 
     2461 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga 
     2521 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga 
     2581 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat 
     2641 ttttgatgat atcctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa 
     2701 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc 
     2761 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg 
     2821 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg 
     2881 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt 
     2941 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt 
     3001 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc 
     3061 acggagtacc gggcgccgtc caggcacctc gattagttct cgaggatcca tatcagcttc 
     3121 tctatttcag ctgcaagaaa ttactaagac acttgaaaat atacatttag aagaaaatgt 
     3181 gtgacgaaag agatctaatt tgtataacca ccatctttct aataactatt aggttggtgc 
     3241 gaacataatt gcagttttta tcattacttt taatggcaaa aactgtaatt acttttgcac 
     3301 caacctaata ttttagtgtg catttggaaa taaatgcacg taaaaagtaa ctaaatcaat 
     3361 tcaaaactta ttctcagatt tgcttggagc cttctataaa gagtgatatc acattcagct 
     3421 agctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag 
     3481 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc 
     3541 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg 
     3601 gttcaaagtt tttttcttcc atttcaggtg tcgtgaaaac tacccctcta gagtcgagct 
     3661 accggtcgcc accatggtga gcaagggcga ggaggataac atggccatca tcaaggagtt 
     3721 catgcgcttc aaggtgcaca tggagggctc cgtgaacggc cacgagttcg agatcgaggg 
     3781 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg 
     3841 tggccccctg cccttcgcct gggacatcct gtcccctcag ttcatgtacg gctccaaggc 
     3901 ctacgtgaag caccccgccg acatccccga ctacttgaag ctgtccttcc ccgagggctt 
     3961 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc 
     4021 ctccctgcag gacggcgagt tcatctacaa ggtgaagctg cgcggcacca acttcccctc 
     4081 cgacggcccc gtaatgcaga agaagaccat gggctgggag gcctcctccg agcggatgta 
     4141 ccccgaggac ggcgccctga agggcgagat caagcagagg ctgaagctga aggacggcgg 
     4201 ccactacgac gctgaggtca agaccaccta caaggccaag aagcccgtgc agctgcccgg 
     4261 cgcctacaac gtcaacatca agttggacat cacctcccac aacgaggact acaccatcgt 
     4321 ggaacagtac gaacgcgccg agggccgcca ctccaccggc ggcatggacg agctgtacaa 
     4381 ggacccaccg gtcgccacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga 
     4441 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg 
     4501 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct 
     4561 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc 
     4621 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg 
     4681 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc 
     4741 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca 
     4801 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc 
     4861 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct 
     4921 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac 
     4981 ccgcaagccc ggtgcctgag cggccgcgtc gacaatcaac ctctggatta caaaatttgt 
     5041 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct 
     5101 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat 
     5161 aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg 
     5221 gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac cacctgtcag 
     5281 ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact catcgccgcc 
     5341 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttg 
     5401 tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg gattctgcgc 
     5461 gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc ttcccgcggc 
     5521 ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac gagtcggatc 
     5581 tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga ccaatgactt 
     5641 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa 
     5701 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc 
     5761 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa 
     5821 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga 
     5881 gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt tcatgtcatc 
     5941 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt 
     6001 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag 
     6061 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg 
     6121 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc 
     6181 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc 
     6241 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta 
     6301 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt 
     6361 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc 
     6421 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc 
     6481 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt 
     6541 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc 
     6601 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct 
     6661 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat 
     6721 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc 
     6781 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc 
     6841 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg 
     6901 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca 
     6961 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata 
     7021 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga 
     7081 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc 
     7141 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg 
     7201 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc 
     7261 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat 
     7321 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact 
     7381 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat 
     7441 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga 
     7501 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc 
     7561 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga 
     7621 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag 
     7681 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc 
     7741 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt 
     7801 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct 
     7861 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg 
     7921 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg 
     7981 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca 
     8041 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga 
     8101 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa 
     8161 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga 
     8221 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt 
     8281 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt 
     8341 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat 
     8401 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct 
     8461 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca 
     8521 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag 
     8581 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc 
     8641 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga 
     8701 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca 
     8761 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag 
     8821 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg 
     8881 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct 
     8941 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt 
     9001 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg 
     9061 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc 
     9121 gcgcaattaa ccctcactaa agggaacaaa agctggagct gc 
//
